【摘要】 目的研究人端粒酶逆转录酶(hTERT)mRNA在喉鳞状细胞癌中和病理分级、临床参数的关系以及和c-myc的相关性。方法用原位杂交法检测40例喉鳞状细胞癌中hTERT mRNA的表达,用免疫组织化学法检测c-myc蛋白。结果喉正常组织、不典型增生、鳞癌组织中hTERT mRNA的阳性率分别为0%(0/10)、10%(1/10)、80%(32/40),喉鳞癌中hTERT mRNA的阳性率明显高于正常组织(P<0.05)和不典型增生(P<0.05),hTERT mRNA水平与c-myc的表达呈显著相关(r=0.5422,P<0.01)。喉鳞癌中hTERT mRNA表达水平与病理分级不相关(P>0.05),与年龄、性别、TNM分期和淋巴结转移情况不相关(P>0.05)。结论喉鳞癌中hTERT mRNA的阳性率明显高于正常组织和不典型增生,有助于喉部良恶性病变的鉴别,c-myc的过度表达可能是喉鳞癌端粒酶活性升高的一个重要机制。更多还原

【Abstract】 Purpose To study the expression of human telomerase reverse transcriptase(hTERT) mRNA and its relationship with c-myc in laryngeal squamous cell cancer(LSCC). Methods Expression of hTERT mRNA was examined by in situ hybridization;expression of c-myc was examined by immunohistochemistry in 40 cases of LSCC. Results Positive rates of hTERT mRNA in normal laryngeal tissue,laryngeal hyperplasia and LSCC were 0%(0/10),10%(1/10) and 80%(32/40),respectively.The prevalence and intensity of hTERT mRNA expression were much greater in LSCC than those in normal laryngeal tissue or laryngeal hyperplasia(P<0.05).the positive correlation between hTERT mRNA and c-myc was significant(P<0.01).The expression level of hTERT were not correlated with age,gender,lymph node metastasis,TNM category and clinicopathological stage(P>(0.05)). Conclusions Level of hTERT mRNA was obviously higher than laryngeal normal tissues and hyperplasia.Semi-quantitative detection of hTERT mRNA expression in situ is helpful in differential diagnosis of malignant tissues and non-malignant tissues of larynx.Overexpression of c-myc may play a role in the transcriptive activation of hTERT gene in LSCC.更多还原