【摘要】 通过静电自组装方法,在磁性纳米颗粒表面依次修饰带正电的聚二甲基二烯丙基氯化胺(Poly(diallyldimethylammoniumchloride),PDADMAC)和带负电的聚丙烯酸(Poly(acrylicacid),PAA),获得羧基化的磁性纳米复合粒子。进而将PAA上的羧基在1-乙基-3(3-二甲基胺基丙基)碳二亚胺盐酸盐(1-ethyl-3(3-dimethylaminopropyl)-carbodiimide,EDC)活化下与多肽LyP-1(CGNKRTRGC)上的氨基发生反应,从而使多肽LyP-1成功地连接到磁性颗粒表面,获得的磁性多肽纳米颗粒可靶向分离肺腺癌SPCA-1细胞,但不识别正常人胚肾293细胞。更多还原

【Abstract】 Carboxylic magnetic nanocomposites have been prepared by depositing positive charged poly(diallyldimethylammonium chloride) (PDADMAC) and negative charged poly(acrylic acid) (PAA)onto the surfaces of Fe3O4 nanoparticles using a layer-by-layer electrostatic self-assembly approach. LyP-1 peptide(CGNKRTRGC) have been successfully conjugated to the carboxylic magnetic nanocomposites using 1-ethyl-3(3-dimethylamino propyl)-carbodiimide (EDC) as a cross-linking reagent. The bioconjugates obtained could specifically separate lung adenocarcinoma cells SPCA-1, but couldn’t recognize normal human embryo kidney cells 293.更多还原