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SRAP、ISSR技术的优化及在甘蓝类植物种子鉴别中的应用

Optimization of SRAP & ISSR Technology and Its Application in the Identification of Seeds of Brassica oleracea L.

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【作者】 刘冲葛才林任云英陈锦秀杨晓锋薄天岳

【Author】 LIU Chong 1,2 ,GE Cai-Lin2,REN Yun-Ying1,CHEN Jin-Xiu1,YANG Xiao-Feng1 and BO Tian-Yue 1* 1 Horticultural Research Institute, SAAS, Shanghai Key Lab of Protected Horticultural Technology, Shanghai 201106,China2 Agricultural College of Yangzhou University, Yangzhou 225009,China

【机构】 上海市农业科学院园艺研究所 上海市设施园艺技术重点实验室扬州大学农学院扬州225009上海市农业科学院园艺研究所上海市设施园艺技术重点实验室上海201106

【摘要】 将SRAP与ISSR2种分子标记技术应用于8种甘蓝类植物(BrassicaoleraceaL.)的种子鉴别中。先以甘蓝(Brassicaoleraceavar.capitata)基因组DNA为模板,通过对SRAP、ISSR反应体系中各影响因素的逐一筛选,优化了甘蓝类植物SRAP、ISSR反应体系。进而采用30个SRAP引物组合和15个ISSR引物对白甘蓝、皱叶甘蓝、红甘蓝、羽衣甘蓝、花椰菜、青花菜、抱子甘蓝、球茎甘蓝的基因组DNA进行了PCR扩增,结果表明M3-E5与M4-E5两个SRAP引物组合可以在8种甘蓝类植物之间显示较高的多态性;844和888两个ISSR引物也可在8种甘蓝类植物之间产生很好的多态,特别是844引物单独应用即可区分所有材料。

【Abstract】 In this study, the molecular marker technology of SRAP and ISSR were applied in rapid identification of seeds from eight species of Brassica oleracea L. Firstly, using the genomic DNA of cabbage as template, SRAP and ISSR reaction systems were optimized through testing every factor, respectively, that affects PCR amplification. Then, using the optimized reaction systems, 30 SRAP primer pairs and 15 ISSR primers were applied to amplify genomic DNA of cabbage, savoy, purple cabbage, borecole, cauliflower, broccoli, Brussels sprouts, and kohlrabi The results showed that high polymorphisms were exhibited among the eight species of Brassica oleracea L. by SRAP primer pairs of M3-E5 and M4-E5, as well as ISSR primers of 844 and 888, especially primer 844 which can identify all eight materials efficiently.

【关键词】 甘蓝类植物SRAPISSR反应体系鉴别
【Key words】 Brassica oleracea L.SRAPISSreaction systemidentify
【基金】 上海市科委基础性研究计划项目(No.03JC14060);上海市农委科技兴农重点攻关项目(No.2004-2-5)。~~
  • 【文献出处】 生物工程学报 ,Chinese Journal of Biotechnology , 编辑部邮箱 ,2006年04期
  • 【分类号】S635
  • 【被引频次】46
  • 【下载频次】437
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