【摘要】 现已证明,在肿瘤发生过程中,司职抗原呈递的树突状细胞的功能存在缺陷和紊乱,从而导致了免疫系统的显著受抑和肿瘤的免疫逃逸.为了探索肿瘤的免疫逃逸机制,利用生物物理学和微观流变学的方法研究了肿瘤来源因素对树突状细胞(dendriticcells,DCs)分化过程的影响.发现来源于肿瘤微环境的细胞因子等成分,导致DCs的渗透脆性增加、细胞膜脂流动性显著下降,而且细胞的转录水平和能量状态也明显改变,导致DCs的抗原摄取能力和活化幼稚T细胞的能力显著下降.所以,DCs的微观流变特性的改变也许是肿瘤免疫逃逸机制的一个方面.更多还原

【Abstract】 The defects and dysfunctions in antigen-presenting dendritic cells have been shown during carcinogenesis leading to human immune system can be markedly suppressed by cancer. To explore the mechanisms by which cancers escape from immune recognition, the influences of tumor microenvironment on differentiation of dendritic cells (DCs), which play an important role in tumor immunology, were studied by biophysical and microrheological methods. It was found that the components derived from tumors caused the increases in osmotic fragility and decreases in membrane fluidity of DCs, moreover, the gene transcriptional levels and energy statuses of cells were also transformed, leading to impaired capabilities of antigen uptaking and deteriorated stimulatory capacities to activate na?觙ve T cells. The impaired microrheological properties of DCs may be one of the aspects of immune escape mechanism of tumor.更多还原