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水葫芦生防炭疽菌(Colletotrichum spp.)的分离与生物学性状

Isolation and Biological Characteristics of the Pathogenic Fungus Causing Anthracnose for Controlling Water Hyacinth

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【作者】 丁义顾振芳吴辉辉庄奇佳褚建君

【Author】 DING Yi1,GU Zhen-fang2,WU Hui-hui2,ZHUANG Qi-jia1,CHU Jian-jun1(1.School of Life Science and Technology,Shanghai Jiaotong University,Shanghai 200240; 2.School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 201101,China)

【机构】 上海交通大学生命科学技术学院上海交通大学农业与生物学院上海交通大学生命科学技术学院 上海200240上海201101上海200240

【摘要】 本文研究了光照、温度、pH、湿度和介质对水葫芦生防真菌炭疽菌(Colletotrichum spp.)生长、产孢和分生孢子萌发的影响。结果表明全黑暗和光暗交替处理有利于该菌菌丝的生长和孢子萌发;光照处理对产孢量无显著影响。该菌菌丝生长、产孢和孢子萌发的温度范围为5 ̄35℃;菌丝生长、产孢最适温度25℃;孢子萌发最适温度35℃。该菌生长和产孢的pH范围为5.0 ̄9.5;最适生长pH为5.6 ̄6.0;最适产孢pH6.6、7.5;孢子萌发的pH范围为3.6 ̄8.0,最适pH5.0、6.6。在相对湿度为75% ̄100%和水滴中,孢子均可萌发,最适湿度90% ̄100%。玉米粉、1%蛋白胨等介质对孢子萌发有促进作用。

【Abstract】 The effect of light、temperature、pH、moisture and medium on the mycelial growth、sporulation and sopre germination of anthracnose fungus,a biological control agent for water hyacinth,was tested.For the mycelial growth of this pathogen,the treatment of light was 24 h darkness and 12 h alternative on illumination and darkness,the range of temperature was 5~35 ℃ with the optimal temperature at 25 ℃,the range of pH was 5.0~9.5 with the optimal pH at 5.6 and 6.0.For the sporulation,the treament of light had no effect,the temperature was 5~35 ℃ with the optimal temperature at 25 ℃,the range of pH was 5.0~9.5 with the optimal pH at 6.6 and 7.5.For the spore germination,the treatment of light was 24h darkness and 12 h alternative on illumination and darkness,the range of temperature was 5~35 ℃ with the optimal temperature at 35 ℃,the range of pH was 3.6~8.0 with the optimal pH at 5.0 and 6.6,the relative moisture was 75 %~100 % and drip,and with the optimum at 90 %~100 %,the medium such as cornmeal、peptone could promote the spore germination.

【基金】 上海市科委重大科技攻关项目(N0.03DZ12007)
  • 【文献出处】 上海交通大学学报(农业科学版) ,Journal of Shanghai Jiaotong University(Agricultural Science) , 编辑部邮箱 ,2006年01期
  • 【分类号】S451
  • 【被引频次】9
  • 【下载频次】163
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