【摘要】 观察CD4+CD25+T和CD4+CD25-T细胞的表型和细胞因子的表达。自小鼠脾脏制备单个细胞悬液,分离CD4+T细胞、CD4+CD25+和CD4+CD25-T细胞,进行细胞表面标记,激活后进行细胞内细胞因子染色,利用流式细胞仪在单个细胞水平上分析细胞表面分子、转录因子和细胞因子表达之间的关系。结果:在CD4+T细胞中,约有7.8%的细胞同时表达CD25分子。与CD4+CD25-T细胞相比,CD4+CD25+T细胞CD44的表达略有增加,CD45RB的表达明显下降,CTLA-4和Foxp3明显增加。以同时表达CTLA-4和Foxp3的细胞为主,其次为单独表达Foxp3的细胞。细胞因子的研究结果表明,与CD4+CD25-T细胞相比,CD4+CD25+T细胞IL-2、IFN-γ明显减少,而只产生IL-10的细胞略有增加。CD4+CD25+调节性T细胞无论在表型、转录因子的表达以及细胞因子表达方面均于非调节性T细胞不同。更多还原

【Abstract】 To compare the phenotype and cytokines expression in CD4⁺CD25⁺T and CD4⁺CD25^-T cells,single cell suspensions from mouse spleen were prepared and the CD4⁺、CD4⁺CD25⁺、CD4⁺CD25^-T cells were isolated.The expression of surface molecules,transcriptional factors and cytokines was determined and analysed at the single cell level by FACS.It was found that 7.8% of CD4⁺T cells expressed CD25.Compared with CD4⁺CD25^-T cells,expression of CD44 on CD4⁺CD25⁺T cells increased slightly,CD45RB decreased remarkably,CTLA-4 and Foxp3 increased significantly.The majority of CD4⁺CD25⁺T cells expressed both CTLA-4 and Foxp3,and much less cells expressed Foxp3 alone.The results of intracellular cytokines staining indicated that the frequency of IL-2⁺ and IFN-γ⁺ CD4⁺CD25⁺T cells decreased remarkably,and the frequency of IL-10⁺ CD4⁺CD25⁺T cells increased lightly compared with CD4⁺CD25^-T cells.It is evident that the CD4⁺CD25⁺regulatory T cells differs both in the phenotype and the expressions of transcriptional factors and cytokines,with the CD4⁺CD25^- non-regulatory T cells cells.It was different in phenotype、expression of transcriptional factors and cytokines in CD4⁺CD25⁺T cells and CD4⁺CD25^-T cells.更多还原