【摘要】 目的:研究酪氨酸蛋白激酶抑制剂Genistein诱导人唾液腺腺样囊性癌细胞株SACC-83细胞周期阻滞的分子机制。方法:用Genistein处理体外培养的SACC-83细胞,采用Western印迹技术检测CyclinB1、Cdk1和CyclinD1、Cdk4蛋白的表达,并利用电泳凝胶成像分析软件,对其结果进行量化分析,采用SPSS11.5统计软件对结果进行方差分析。结果:随着Genistein作用时间的延长和浓度的增加,CyclinB1、Cdk1和CyclinD1、Cdk4蛋白的表达明显减少。SACC-83细胞经220μmol/LGenistein作用3d,其CyclinB1、Cdk1和CyclinD1、Cdk4蛋白的表达量分别是对照组的58%、64%和46%、43%,差异非常显著(P<0.01)。结论:Genistein诱导SACC-83细胞周期阻滞于G2/M期,与其下调CyclinB1、Cdk1和CyclinD1、Cdk4蛋白的表达有关。更多还原

【Abstract】 PURPOSE:To investigate the molecular mechanism of cell cycle arrest induced by tyrosine protein kinase inhibitor,genistein,in human salivary adenoid cystic carcinoma cell line SACC-83.METHODS:SACC-83 cells cultured in vitro were treated with genistein,the expressions of CyclinB1,Cdk1,CyclinD1 and Cdk4 proteins were detected with Western blotting,and the results were quantitatively analyzed by FluorChem V2.0 software,statistical analysis was performed with analysis of variance using SPSS11.5 software.RESULTS:With the increase of concentration of genistein and the elongation of time,the expression of CyclinB1,Cdk1,CyclinD1 and Cdk4 proteins was significantly decreased.Treated with 220μmol/L of genistein for 3 days,the expression level of CyclinB1,Cdk1,CyclinD1 and Cdk4 proteins was 58%,64%,46% and 43% of the control group,respectively(P<0.01).CONCLUSION:The cell cycle G2/M arrest induced by genistein in human salivary adenoid cystic carcinoma cell line SACC-83 may be associated with the downregulations of CyclinB1,Cdk1,CyclinD1 and Cdk4 protein expressions.Supported by Scientific Research Plan of Education Bureau of Liaoning Province(05L533).更多还原