【摘要】 目的探讨生长抑素(SST)类似物奥曲肽逆转肝癌细胞多药耐药可能的机制。方法应用M TT法分析肝癌细胞对化疗药物的敏感性;RT-PCR、流式细胞术检测肝癌细胞多药耐药基因多药耐药糖蛋白1(M DR1)、多药耐药相关蛋白2(M RP2)mRNA及其蛋白质的表达。结果奥曲肽联合化疗药物可以显著降低化疗药物的IC50。肝癌细胞有生长抑素受体2(SSTR2)、生长抑素受体3(SSTR3)、M DR1、M RP2的表达,奥曲肽可显著降低肝癌细胞表面M DR1、M RP2的表达。结论SST可与肝癌细胞表面的SSTR结合,降低其表面M DR1、M RP2的表达,使细胞内细胞毒药物浓度增加,从而逆转肝癌细胞多药耐药。更多还原

【Abstract】 [Objective] To investigate the possible mechanism of octreotide reverses multidrug resistance and the effect of octreotide chemosensitizes hepatoma cells.[Methods] The expression of the MDR₁,MRP₂ mRNA and protein were analyzed by RT-PCR and flow cytometer respectively.The cytotoxic effect of epirubincin,carboplatin,hydroxyl-camptothecin and 5-fluorouracil was analyzed by MTT assay.[Results] The 50% inhibitory concentration of cytotoxic agents was significantly reduced when combination with octreotide.There were SSTR₂,SSTR₃,MDR₁ and MRP₂ expression in hepatoma cells,the expression of MDR₁ and MRP₂ on the surface of hepatoma cells reduced significantly after octreotide treatment.[Conclusions] Octreotide can chemosensitize hepatoma cells by inhibiting the expression of the MDR₁ and MRP₂ gene on the surface of hepatoma cells.更多还原