【摘要】 目的探讨氧化低密度脂蛋白(Ox-LDL)对人血管内皮细胞血小板内皮细胞黏附分子-1(PECAM-1)mRNA表达的影响。方法体外培养人脐静脉内皮细胞(HUVEC),采用铜离子氧化24 h制备Ox-LDL。取HUVEC以100 m g/L Ox-LDL分别孵育0.5、1、1.5、2 h,另取HUVEC分别用100 m g/L LDL和0、50、100、200m g/L Ox-LDL孵育1.5 h,均采用RT-PCR技术检测细胞中PECAM-1基因的转录水平。结果Ox-LDL除正常LDL所含成分外,还含有胆固醇亚油酸酯的过氧化特异斑点X和甲基脂肪酸。Ox-LDL作用0.5 h时PECAM-1mRNA表达水平最低;随时间延长,表达水平逐渐升高,与0.5 h时比较,P<0.05;至2 h时,PECAM-1表达水平有所回落,但与0.5 h时比较,P<0.05。0、50、100、200 m g/L Ox-LDL作用1.5 h,PECAM-1表达水平逐渐升高,以100 m g/L时表达水平最高。100 m g/L LDL作用1.5 h时,PECAM-1表达水平无明显变化。结论Ox-LDL可在转录水平上调血管内皮细胞PECAM-1基因表达,从而促进血管内皮细胞高水平表达PECAM-1。更多还原

【Abstract】 [Objective] To investigate the effect of oxidized low-density lipoprotein(Ox-LDL) on expression of PECAM-1 mRNA in human endothelial cells.[Methods] LDL was oxidized in the presence of Cu~2+ for 24 hours,and lipid component of Ox-LDL was then determined by thin layer chromatography(TLC).Human umbilical vein endothelial cells(HUVECs) were incubated with 100 mg/L Ox-LDL for 0.5,1,1.5,2 hours;also with 100 mg/L LDL and 0,50,100,200 mg/L Ox-LDL for 1.5 hours,then transcription of PECAM-1 gene in HUVECs was determined with reverse transcription polymerase chain reaction(RT-PCR).[Results] Hydroperoxidized cholesteryl linoleate(Spot X) and methylated fatty acid were produced in Ox-LDL.Ox-LDL increased PECAM-1 gene transcription significantly in HUVECs in time-dependent manner,but decreased a little at 2 h(P<0.05,compared to 0.5 h).Incubation with 0,50,100,200 mg/L Ox-LDL for 1.5 hours significantly increased PECAM-1 transcription,which reached to peak point at 100 mg/L.PECAM-1 transcription level did not changed by 100 mg/L LDL.[Conclusion] Ox-LDL up-regulates the expression of PECAM-1 gene at transcription level and thereby probably increase the expression of PECAM-1 protein in HUVECs.更多还原