【摘要】 目的探讨前列腺癌(PC a)组织中血管生成与细胞外基质(ECM)的相关性,及其与肿瘤生物学行为之间的关系。方法应用免疫组织化学LSAB法检测38例PC a患者癌组织中微血管密度(M VD)以及Ⅳ型胶原(COLⅣ)、纤维连接蛋白(FN)、层粘连蛋白(LN)等ECM。结果高分化癌组织中M VD明显低于低分化和未分化者(P<0.05);有淋巴结转移的癌组织中M VD明显高于无转移者(P<0.001);高分化癌组织中COLⅣ、FN、LN呈连续型分布的百分率明显高于未分化者;有淋巴结转移者COLⅣ及FN呈连续型分布的百分率明显低于无转移者。COLⅣ、FN、LN呈连续型分布者的M VD均明显低于呈非连续型分布者,呈负相关。结论血管生成和ECM在PC a侵袭和转移过程中均起重要作用,两者关系密切,相互影响、相互作用。更多还原

【Abstract】 [Objective] To study the correlation between angiogenesis and extracellular matrix in human prostate cancer and their relationship with the biological behavior of tumor.[Methods] Microvessel density(MVD), laminin(LN) expression,type Ⅳ collagen(COLⅣ),fibronectin(FN) in 38 cases of invasive prostatic cancer were detected with antibodies to F8-RAg,LN,COLⅣ,FN using immunohistochemical techniques(LSAB).[Results] Microvessel density in highly differentiated prostatic cancer was significantly lower than that in poorly differentiated and undifferentiated prostatic cancer(P<0.05).Microvessel density in prostatic cancer with lymph node metastasis was significantly higher than that in node-negative cancer(P<0.001).It was found that expressions of LN,COLⅣ and FN were related to the degree of tumor differentiation and lymph node,being positive or negative metastasis.Continuous(linear) was displayed in the most highly differentiated or node-negative carcinoma,while interrupted(linear) or fragmentary in the most poorly differentiated,undifferentiated or node-positive carcinoma.And microvessel density in prostatic cancers in which LN,COLⅣ or FN appeared continuously was significantly lower than that appearing discontinuously.[Conclusion] Angiogenesis and ECM both play important roles in invasion and metastasis of human prostatic cancer.更多还原