【摘要】 目的探讨ESAT-6体外诱导的γ-干扰素释放反应(简称ESAT-6反应)在人免疫缺陷病毒(H IV)合并结核杆菌(TB)感染中的诊断价值。方法选择H IV(+)TB(+)患者78例,H IV(+)TB(-)患者60例,H IV(-)TB(+)患者30例,健康对照者20例,用全血测定法检测ESAT-6反应。结果ESAT-6反应在H IV(+)患者合并和非合并结核患者中有显著性差异(P<0.01),ESAT-6反应对H IV(+)TB(+)患者检出率明显高于PPD皮试、PPD诱导的γ-干扰素反应。结论我们建立的ESAT-6反应测定法简便、灵敏、特异性高,对H IV合并结核感染患者具有较好的诊断价值。更多还原

【Abstract】 [Objective] To explore the value of interferon γ(IFN-γ) response induced by ESAT-6 in vitro in mycobacterium tuberculosis(TB) diagnosis among human immunodeficiency virus(HIV) seropositive patients.[Methods] 78 HIV(+)TB(+) patients,60 HIV(+) TB(-) patients,30 HIV(-) TB(+) patients and 20 normal controls were selected.Heparinized whole blood was incubated at 37℃ for 16-20 hours after ESAT-6 was added.IFN-γ was detected by ELISA method. [Results] The occurrence rate of IFN-γ response induced by ESAT-6 in vitro was much higher than that of PPD skin test or PPD-induced IFN-γ response.There was a significant difference between the HIV(+) TB(+) group and the HIV(+) TB(-) group compared to control groups(P<0.01).[Conclusion] This work set up the method of ESAT-6-induced IFN-γ response in vitro.This method is simple,sensitive and specific.It has some value in diagnosis of HIV coinfected TB.更多还原