【摘要】 目的研究山东汉族人群血小板特异性抗原(HPA)1~5和15系统基因多态性分布特点。方法采用PCR-序列特异性引物(PCR-SSP)技术对234例无血缘关系,汉族,血小板志愿捐献者进行HPA-1~5和HPA-15系统基因分型,计算等位基因频率、基因型频率并与其他种族、地区人群相关资料比较。结果等位基因频率由高到低依次为HPA-4a、-1a、-5a、-2a-、3a、-15a、-15b、-3b-、2b-、5b-、1b,未检到HPA-4b。基因型频率由高到低依次为HPA-4a4a、-1a1a、-5a5a、-2a2a-、15a15b-、3a3b、-3a3a-、15a15a、-3b3b、-15b15b-、2a2b、-5a5b-、1a1b,未检到HPA-1b1b-、5b5b和HPA-2b2b。与报道的东方人群比较,有显著性差异的系统有HPA-2、-3-、5(P<0.005);与西方人群比较,HPA-1、-2、-3、-5系统有显著性差异(P<0.005),HPA-4无种族间显著性差异。HPA-15系统的基因分布与越南、德国、奥地利人近似,而与印地安人有较大差异(P<0.005)。结论HPA存在明显的种族和地域性差异。山东汉族人发生HPA同种免疫的可能风险抗原基因主要有HPA-3a,-3b,-2b,-5b,-1b,其次为HPA-15a、-15b。更多还原

【Abstract】 Objective: To investigate the allele polymorphism of HPA-1-5 and HPA-15 systems in Hans of Shandong Province in China. Methods: A genetic method of polymerase chain reaction-sequence specific primers(PCR-SSP) was used to determine genotype of HPA-1～5and HPA-15 in 234 blood platelet donors in Shandong Blood Center.The gene frequencies and genotype frequencies were evaluated and compared with those in other populations.Results: The alleles listed from high to low frequency were HPA-4a,-1a,-5a,-2a,-3a,-15a,-15b,-3b,-2b,-5b and-1b,HPA-4b wasn’t detected.While genotypes were HPA-4a4a,-1a1a,-5a5a,-2a2a,-15a15b,-3a3b,-3a3a,-15a15a,-3b3b,-15b15b,-2a2b,-5a5b and-1a1b;HPA-1b,-5b and HPA-2b homozygote individuals were not found.The frequency distributions of HPA-1～5 were found to be comparable with those reported in oriental populations,but in HPA-2,-3,-5,there was significant difference(P<0.005).Compared with western populations,significiant difference(P<0.005) was found in HPA-1～3 and-5.As to the frequency distributions of HPA-15,apart from Amazon Indians(P<0.005),there were no significant difference between Chinese and Vietnamese,Germans,Austrians respectively.Conclusion: This study confirmed the genetic and original difference of HPA and indicated that the most possible risk HPA alleles which may induce HPA alloimmunization were HPA-3a,-3b,-2b,-5b,-1b and HPA-15a,-15b.更多还原