【摘要】 目的研究生长分化因子-5(GDF-5)在大鼠肢体早期发育过程中的表达规律,探讨GDF-5调控肢体发育的相关机制。方法采用半定量RT-PCR和W estern b lotting分别检测GDF-5在大鼠胚胎早期发育过程中肢芽组织mRNA和蛋白表达的变化。结果大鼠胚胎肢体发育的早期阶段GDF-5 mRNA呈持续表达,其中在胚胎13、14d(E 13、E 14)呈高表达,继而表达减弱,GDF-5蛋白表达规律与mRNA变化趋势一致。结论GDF-5在大鼠胚胎早期肢体发育中对调控软骨发育和关节形成有很重要的作用。更多还原

【Abstract】 Objective: To investigate the expression trend and effect of growth differentiation factor-5(GDF-5) during appendicular skeletal and joint development in rat.Methods: The expression of GDF-5 mRNA and protein of rat fetal limb buds were detected in embryonic days by RT-PCR and Western blotting respectively.Results: During early stage of limbs development,the expression of GDF-5 mRNA was constant,stepped up at embryonic day 13 and 14(E13 and E14),subsequently dropped at E15 and E16.The expression of GDF-5 protein had a similar change with mRNA during limbs development.Conclusion: GDF-5 plays an important role in limbs early chondrogenesis and joint formation.更多还原