【摘要】 目的探讨噬菌体展示技术在鼠CD 137配体(CD 137L)活性研究中的应用。方法用PCR法扩增出鼠CD 137L胞外区,用噬菌体展示技术展示其CD 137L;EL ISA方法检测噬菌体CD 137L的抗原性,并分3次注射到大鼠体内,取血清检测大鼠体内多克隆抗体水平。结果PCR方法成功扩增出鼠CD 137L胞外区;获得重组子pCom b3H-CD 137L,成功展示在噬菌体上;噬菌体CD 137L可与抗CD 137L特异性结合;大鼠体内产生了CD 137L的多克隆抗体。结论噬菌体展示技术用于鼠CD 137L活性的研究,可提高制备特异性抗体的成功率,得到特定结构和功能的蛋白质。更多还原

【Abstract】 Objective:To explore the application of phage display technique in study of rat CD137L activity.Methods: Amplify the extracellular region of mouse CD137L by PCR method,dispay it through phage display technique,and detect its antigenicity by ELISA.CD137L was injected into rats by three times and the level of polyclonal antibody in the rats sera was detected.Results: The extracellular region of mouse CD137L was successfully amplified.The recon pComb3H-CD137L was obtained and successfully displayed in the phage.The phage CD137L could specifically bind with anti-CD137L.CD137L polyclonal antibody was found in the rats.Conclusion: Phage display technique in the study of mouse CD137L activity can elevate the achievement radio of preparation for polyclonal antibody and can obtain proteins with definite structure and function.更多还原