【摘要】 目的:观察红霉素对上皮细胞凋亡的影响,探讨上皮细胞凋亡在鼻息肉组织形成中的意义。方法:将30例鼻息肉及6例下鼻甲上皮细胞均分成2组,1组加红霉素,培养原代上皮细胞,另1组不加红霉素,1、35、d时用TUNUL法检测细胞凋亡。结果:红霉素组培养1、3、5 d的上皮细胞凋亡指数分别为(33.23±6.50)%、(38.21±7.22)%和(52.63±7.86)%,不加红霉素组为(31.02±5.60)%、(32.13±7.15)%和(39.64±7.48)%,培养5 d的上皮细胞凋亡指数两组间差异有统计学意义(P<0.05)。结论:红霉素有明显的促鼻息肉上皮细胞凋亡的作用。更多还原

【Abstract】 Objective: To study the effect of erythromycin on apoptosis in nasal polyps.Methods: Epithelial cells from 30 nasal polyps and 6 inferior turbinates were cultured in Dulbecco eagle and Ham F12(1:1),which were divided into two groups,one with erythromycin,and the other without.Apoptosis was detected by terminal deoxynucleotidyl transferasemediated dUTP nickendlabeling.Results: The apoptosis index(AI) of epithelial cells in nasal polyps after cultured for 1,3,5 days was(33.23±6.50)%,(38.21±7.22)% and(52.63±7.86)% respectively.The AI of epithelial cells in inferior turbinate was(31.02±5.60)%,(32.13±7.15)% and(39.64±(7.48))% respectively.There was a significant difference between the two groups at day 5 after culture(P<0.05).Conclusion: Erythromycin promotes apoptosis of epithelial cells from nasal polyps.更多还原