【摘要】 目的建立高效液相色谱法测定通窍鼻炎片中5-O-甲基维斯阿米醇苷的含量。方法Kromassil C18色谱柱,5μm,250×4.6mm,甲醇-水-四氢呋喃(38∶62∶1)为流动相,流速为1.0ml.min-1,检测波长254nm。结果本法线性关系良好(r=0.9998)。平均加样回收率100.07%,RSD=1.39%(n=5)。结论本法简便易行,结果准确,重现性好。可用于通窍鼻炎片的质量控制。更多还原

【Abstract】 OBJECTIVE To establish a HPLC method for determination of 5-O-Methylvisammioside in Tongqiao Biyan Tablets.METHODS The Kromassil C₁₈ column（5μm,250×4.6mm） was used,the mobile phase was methanol-water-tetrahydrofuran（38∶62∶1）.Flow rate was 1.0ml·min^-1.Detection wavelength was at 254nm.RESULTS The method had a good linear dependence（r=0.9998）.The average recovery was 100.07%（RSD=1.39%,n=5）.CONCLUSION The method is simple,rapid,accurate and reliable,and can be used to control quality of Tongqiao Biyan Tablets.更多还原