【摘要】 目的:探讨葡萄籽原花青素(grape seed proanthocyanidin extract,GSPE)对兔实验性心肌再灌注损伤(myocardial reperfusion injury,MRI)诱发的心肌细胞凋亡的保护作用。方法:建立兔在体心肌再灌注损伤模型,24只兔随机平分为4组:假手术对照组(A)、缺血再灌注模型组(B)分别用生理盐水灌胃,低剂量GSPE治疗组(C)用GSPE(100 mg/kg)灌胃,高剂量GSPE治疗组(D)用GSPE(200 mg/kg)灌胃,共喂养21 d。各组测定再灌注前后不同时间血清心肌肌酸激酶同工酶MB(CK-MB)、超氧化物歧化酶(SOD)、丙二醛(MDA)含量,对照观察各组兔心肌超微结构变化及心肌细胞凋亡情况。结果:与B组相比,C、D组的血清CK-MB和MDA显著降低(P<0.05,P<0.01),血清SOD明显升高(P<0.01);病理分析显示,C、D组兔心肌细胞损伤明显减轻,心肌细胞凋亡的数目明显减少,D组凋亡指数(AI)明显降低(P<0.01)。结论:GSPE在减轻兔MRI的同时,对其引起的细胞凋亡有明显的保护作用,可能是其抗MRI的机制之一。更多还原

【Abstract】 Objective: To study the effect of grape seed proanthocyanidin extract(GSPE) on the apoptosis of myocardial cells induced by myocardial reperfusion injury(MRI) in rabbits.Methods: Twenty-four male New Zealand rabbits were divided into four groups at random: control,model,low dose and high dose groups.After 21 days of treatment,serum CK-MB,SOD and MDA of the rabbits of each group were detected at different times before and after reperfusion,the ultra-structure of myocardium and the apoptosis of myocardial cells were observed.Results: The CK-MB and MDA decreased,but SOD increased in GSPE group compared with the model group(P<0.01,P<0.05).Pathological analysis manifested MRI revived markedly in GSPE group.The amount of apoptotic cells and the apoptosis index(AI) reduced significantly in GSPE group(P<0.01).Conclusion: GSPE has a significantly preventive effect on myocardial cell apoptosis induced by MRI,except for its potent antioxidant activity,which may be one of the mechanisms of its anti-MRI effect.更多还原