【摘要】 目的:探讨高脂诱导肥胖模型大鼠血清瘦素、肿瘤坏死因子-α(TNFα-)、白细胞介素6(IL-6)的水平及其在胰岛素抵抗中的作用。方法:大鼠高脂饮食8周后,以体重比对照组重30 g为标准,分出肥胖大鼠,继续饲养4周,空腹麻醉下,心脏取血,分离血清。葡萄糖氧化酶法测血清血糖;放射免疫法(RIA)测血清瘦素、胰岛素;酶联免疫法(ELISA)测血清TNFα-、IL-6水平。以空腹胰岛素/血糖(Insulin/Glu-cose,I/G)比值作为胰岛素敏感指标。结果:与对照组相比,肥胖大鼠空腹胰岛素、I/G比值,瘦素、IL-6水平显著高于对照组(P均<0.05),血清TNFα-水平无差别;肥胖大鼠I/G比值与血清瘦素、IL-6均密切相关(r=0.772,P=0.036;r=0.898,P=0.015),与TNFα-不相关(r=0.341,P=0.254)。结论:肥胖大鼠血清瘦素、IL-6显著增高,参与了肥胖相关的胰岛素抵抗,血清TNFα-未增高,与肥胖相关的胰岛素抵抗无关。更多还原

【Abstract】 Objective: To evaluate the role of serum leptin, TNF-α and IL-6 on insulin resistance in high-fat diet induced obese rats.Methods: After fed with high-fat diet for 8 weeks,the obese rats who were 30g heavier than controls were separated and fed for another 4 weeks.The blood samples were drawed by heart puncture.The serum levels of glucose were measured by glucose oxide-peroxidase method,the levels of leptin and insulin by RIA,and the levels of TNF-α,IL-6 by ELISA.The ratio of insulin versus glucose(I/G) was used as an indicator for insulin resistance.Results: Compared with the controls,the serum levels of leptin,IL-6 and I/G were higher in the obese rats,but the levels of TNF-α were not different between two groups;The levels of leptin and IL-6,but not TNF-α,were closely related to the parameters evaluating insulin resistance in obese rats.Conclusions: The serum levels of leptin and IL-6 are increased in high-fat diet induced obese rats and contribute to insulin resistance.The levels of TNF-α in obese rats are neither statistically increased nor related to insulin resistance.更多还原