【摘要】 目的:研究细胞毒性T淋巴细胞相关抗原-4融合蛋白(CTLA-4Ig)诱导再生障碍性贫血(AA)患者骨髓T淋巴细胞免疫耐受的作用。方法:采用免疫荧光标记法、流式细胞术分析CTLA-4Ig阻断前后AA患者骨髓T淋巴细胞表型CD3+、CD4+、CD8+、CD25+、CD4+CD25+和CD8+CD25+的变化。结果:再障试验组与对照组相比,重型再障(SAA)和非重型再障(NSAA)患者骨髓CD3+、CD25+比值、CD4+和CD8+细胞中CD25+细胞比值均明显减低(P<0.05),CTLA-4Ig对重型再障的抑制作用高于非重型再障,对CD4+细胞的抑制作用高于CD8+群体。结论:CTLA-4Ig能够抑制再生障碍性贫血患者骨髓局部的异常自身免疫反应,降低骨髓T淋巴细胞对丝裂原的异常反应。更多还原

【Abstract】 Objective: To investigate the inhibition of the PHA-induced abnormal bone marrow T lymphocyte proliferation and activation by the CTLA-4Ig fusion protein in vitro in patients with aplastic anemia(AA).Method: Flow cytometry and immunofluorescence were used to analyze the mononuclear cells from bone marrow of the aplastic anemia patients.Result: In contrast to the controls,the expressions of the CD3,CD25,CD25 on CD4~+,CD8~+ lymphocytes were statistically decreased(P<0.05) in both serious and non-serious AA.Conclusion: The CTLA-4Ig fusion protein can induce the T cell tolerance and inhibit the T cell abnormal proliferation and activation in patients with AA.更多还原