【摘要】 目的:探讨褐藻多糖硫酸酯(Fucoidan,FPS)对阿霉素肾硬化大鼠肾脏的保护作用。方法:将32只SD大鼠分成4组:假手术组(SHAM组),阿霉素肾硬化模型组(M组),褐藻多糖硫酸酯治疗组(FPS组),洛汀新治疗组(B组)。8周后观察各组大鼠24 h尿蛋白定量、血尿素氮(BUN)、肌酐(Scr)及肾脏病理改变,免疫组化观察肾脏组织纤连蛋白(FN)、Ⅳ型胶原(ColⅣ)、转化生长因子β1(TGF-β1)的表达。RT-PCR测定肾皮质转化生长因子β1(TGF-β1)和纤溶酶原激活物抑制物(PAI-1)mRNA。结果:FPS能减少尿蛋白、降低BUN和Cr,减轻基质增生和肾小球硬化,且能减少TGF-β1、FN、ColⅣ表达,下调肾皮质TGF-β1P、AI-1 mRNA表达。结论:FPS能减轻阿霉素肾硬化大鼠肾脏损害,对肾脏有保护作用。更多还原

【Abstract】 Objective: To investigate the effects and mechanism of fucoidan on adriamycininduced glomerulosclerosis in rats.Methods: Thirty-two Sprague Dawley rats were randomly divided into sham operation,glomerulosclerosis model,fucoidan treatment,and valsartan groups.The 24-hour urinary protein excretion,renal function were measured after 8 weeks.Renal pathology and immunohistochemistry were assayed to measure the expression of fibronectin（FN）,collagen Ⅳ（Col Ⅳ）,transformor growth factor β₁（TGF-β₁）.RT-PCR was used to examine the TGF-β₁ mRNA and plasminogen activator inhibitor-1（PAI-1） mRNA.Result: The proteinuria,BUN,and serum creatinine declined significantly（P<0.01）,and the expression of FN,Col Ⅳ,TGF-β₁,TGF-β₁ and PAI-1 decreased by fucoidan（P<0.01）.Conclusion: Fucoidan plays an important role on prevention and treatment for glomerulosclerosis.更多还原