【摘要】 目的:观察葡萄籽原花青素(GSPE)对实验性动脉粥样硬化基质金属蛋白酶9(MMP-9)的影响,探讨其抗动脉粥样硬化的可能机制。方法:随机将雄性新西兰兔24只分为三组:正常对照组,饲喂标准颗粒饲料;模型组,饲喂含1%胆固醇的颗粒饲料;干预组,饲喂含1%GSPE和1%胆固醇的颗粒饲料。于实验前及实验的第4、8、12周末经耳中动脉采空腹血,检测血清MMP-9水平。于12周末,留取主动脉作常规病理观察和免疫组化染色。结果:与模型组相比,GSPE干预组病变明显减轻,主动脉MMP-9表达明显减少,血清MMP-9水平明显下降(1.06±0.24vs1.33±0.29,P<0.05)。结论:GSPE具有抗动脉粥样硬化效应,该效应的发挥与其抑制MMP有关。更多还原

【Abstract】 Objective: To observe the effects of grape seed proanthocyanidin extract(GSPE) on matrix metalloproteinase in experimental atherosclerosis rabbits and explore its mechanism.Methods: Twenty-four male New Zealand rabbits were randomized into three groups with 8 in each.The normal control group were fed with standard diet.The model group were fed with standard diet containing 1% cholesterol.The GSPE group were fed with standard diet containing 1% grape seed proanthocyanidin plus 1% cholesterol.Blood samples were drawn from ear middle arteries of rabbits just before the experiment and at the 4th,the 8th and the 12th weekend of the experiment.All the rabbits were fasted for at least eight hours before the blood was drawn.The blood samples was analyzed for the matrix metalloproteinase-9(MMP-9).All the rabbits were sacrificed at the 12th weekend,and the expression of MMP-9 was observed in the thoracic aortic tissue using immunohistochemistry technique.Results: The severity of atherosclerosis in GSPE group was less than that in model group.GSPE lowered the expression of MMP-9 of aortas in model group and the levels of serum MMP-9(1.06±0.24 vs 1.33±0.29,P<0.05).Conclusion: GSPE has the efficacy of anti-atherogenesis,which is associated with its reducing the expression of matrix metalloproteinase.更多还原