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渗透压法测定一次性使用真空采血管中EDTA-K2含量
Determination of EDTA-K2 in Single-use Evacuated Tubes for Blood Specimen Collection By Osmotic Pressure
【摘要】 建立一次性使用真空采血管中EDTA-K2的渗透压测定法。建立EDTA-K2的标准曲线,样品管精密加入公称容量的蒸馏水,使抗凝剂全部溶解后测定,由标准曲线计算出样品管中EDTA-K2的浓度。结果表明:线性范围为0.5～4.0mg/mL,r=0.999,与经典方法比较经t检验,5%的水平上无显著性差异。
【Abstract】 To establish an osmotic pressure method for determination of EDTA-K2 in evacuated tubes for blood specimen collection.A standard curve of EDTA-K2 was established and the distilled water of nominal capacity was added in the test tubes accurately,the additive was dissolved completely and tested,then the concentration of EDTA-K2 from the standard curve was caculated.Result showed that the linear range was 0.5～4.0mg/mL,the regression coefficient was 0.999,compared with classical method,there were no remarkable difference on the 5% level.The method i ssimple,quick,reliable.
【Key words】 EDTA-K2； Assay； Osmotic pressure method； Blood specimen collection； Single-use；
- 【文献出处】 生物医学工程研究 ,Journal of Biomedical Engineering Research , 编辑部邮箱 ,2006年03期
- 【分类号】R194.5
- 【被引频次】2
- 【下载频次】101