【摘要】 以花育22号成熟种子萌发后的下胚轴为外植体,分析了激素配比、外植体萌发日龄、AgNO3、NAA以及不同基因型对花生下胚轴植株再生的影响.结果发现,MS+6-BA(12 mg/L)+NAA(0.8 mg/L)的组合对下胚轴芽的分化较好,萌发4~5 d的外植体用于丛生芽诱导较佳,3 mg/L的AgNO3可提高下胚轴芽的分化率并有效减轻下胚轴的褐化,1 mg/L的NAA利于组培苗生根,组培苗移栽后能正常开花、结实.不同基因型花生的芽诱导率存在差异.更多还原

【Abstract】 The hypocotyls of peanut（Arachis hypogaea L.）huayu No.22 were used as the explants to analyze the influence of plant hormones,seed age,AgNO₃,NAA and genotypes on regeneration frequency.It was shown that MS+6-BA 12?mg/L+NAA（0.8?mg/L） could well induce shoot production of hypocotyls;4～5?d seedlings were suitable for inducing shoots;3?mg/L AgNO₃ were able to increase the frequency of hypocotyls differentiation and inhibit explants browning effectively;1?mg/L NAA could well induce roots of shoots.After transplanted into soil,the plantlets grew well and produced viable seeds in greenhouse.Different genotypes had some difference in percentage of shoot induction of hypocotyls.更多还原