节点文献
增强型绿色荧光蛋白表达对鼠C6细胞生物学特征影响的研究
Effects of Expression of Enhanced Green Fluorencent Protein Gene on C6 Cells
【摘要】 脂质体包裹质粒pEGFP-N1转染C6细胞,经G418筛选,得到稳定表达绿色荧光的C6-EGFP细胞.细胞计数,MTT,流式细胞术分别测定C6细胞在转染绿色荧光蛋白后增殖性,细胞活力以及细胞周期等生物学特性.C6细胞在转染了绿色荧光蛋白基因后细胞增殖性降低,细胞活力降低,细胞周期也发生了一些改变.
【Abstract】 C6 cells were transfected with pEGFP-N1 by lipofectamine Plus.Stable EGFP-expressing cells were isolated with G418.Proliferation,activity and cell cycle of C6-EGFP were evaluated through cell-counting, MTT and Flowcytometry.As C6 cells transfected with pEGFP-N1,the proliferation and activity effects decreased and the cell cycle changed.
【关键词】 增强型绿色荧光蛋白;
转染;
C6细胞;
MTT;
流式细胞术;
【Key words】 EGFP; transfection; C6 cells; MTT; flow cytometry;
【Key words】 EGFP; transfection; C6 cells; MTT; flow cytometry;
- 【文献出处】 四川大学学报(自然科学版) ,Journal of Sichuan University(Natural Science Edition) , 编辑部邮箱 ,2006年03期
- 【分类号】R739.4
- 【被引频次】4
- 【下载频次】179