【摘要】 为建立家蚕转基因研究中切实可行的外源基因导入方法、分别用显微注射法、精子介导法、脂质体法和压力渗透法将含有绿色荧光蛋白(gfp)基因的转座子载体和辅助质粒转入到家蚕的受精卵中。在后代中检测到发绿色荧光的蚕茧,用PCR方法检测到后代个体染色体中含有gfp基因,并比较了上述几种方法的优缺点,为进一步进行转基因家蚕的研究奠定了基础。更多还原

【Abstract】 In order to find efficient foreign gene transfer methods in transgenic silkworm research,the transposon vector containing green fluorescent protein gene(gfp) and helper plasmid were transferred into the fertilized eggs of silkworm by the method of pressured permeation,liposome-mediated gene transfer,sperm-mediated gene transfer and microinjection.In the next generation,the green fluorescence can be detected on silkworm cocoon under UV light and gene of gfp in the chromosomes can be detected by PCR.The advantages and disadvantages of these methods have been compared.This is the base for the further research on transgenic silkworm.更多还原