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仙人掌多糖提取过程中三种脱蛋白方法的比较研究

Comparison study on three methods of removing protein from extracted polysaccharide of cactus

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【作者】 史德芳杨洋孙晓雪任红韩淑琴

【Author】 SHI De-fang,SUN Xiao-xue,REN Hong,HAN Shu-qin (College of Light Industry and Food Engineering,Guangxi University,Nanning 530004,Guangxi,China) YANG Yang (College of Life Science,Guangxi University,Nanning 530004,Guangxi,China)

【机构】 广西大学轻工与食品工程学院广西大学生命科学学院广西大学轻工与食品工程学院 广西南宁530004广西南宁530004

【摘要】 用水提取的仙人掌多糖常含有蛋白质,实验以多糖损失率和脱蛋白率为衡量指标,选用了3种方法:Sevag法、三氯乙酸法和酶法对仙人掌多糖提取物进行脱蛋白处理。实验结果表明:酶法、三氯乙酸法和Sevag法的脱蛋白率分别为89.6%、56.2%和45.5%,多糖损失率分别为7.2%、9.5%和13.6%。经比较认为:酶提取法是替代常规方法的一种有效方法。

【Abstract】 In order to obtain purified polysaccharide from cactus, the effects of three methods of removing protein from the extracted polysaccharide of cactus were studied by comparing the percentage of decomposed polysaccharide (PDP) and removed protein (PRP). The results showed that the PRP for the method using enzyme, using CCl3COOH and using Sevag were 89.6%, 56.2% and 45.5%,respectively;and the PDP for the three methods were 7.2%,9.5% and 13.6%, respectively. Through the comparison,it was concluded that the enzymatic removal of protein is an effective alternative to the conventional method.

【关键词】 仙人掌多糖脱蛋白
【Key words】 cactuspolysaccharideremoval of proteinenzyme
  • 【文献出处】 饮料工业 ,The Beverage Industry , 编辑部邮箱 ,2006年10期
  • 【分类号】TS201.1
  • 【被引频次】12
  • 【下载频次】202
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