【摘要】 应用化学沉淀剂将霍乱弧菌沉淀聚集于水体下层,加上缓冲溶液,保持其弱碱生长环境,抑制其它细菌的生长,来快速提高霍乱弧菌的检出率。样品离心后,以NaCO3为介质,FeSO4为沉淀剂,在一定的比例下,沉淀水中的霍乱弧菌,弃去上层清液,底液加入碳酸盐缓冲液。用等量双倍浓碱性蛋白胨水增菌、分离、鉴定。此方法无需二次增菌,比常规增菌法缩短近一半的工作周期,且可降低试剂成本,检测灵敏度要高于常规检测法。更多还原

【Abstract】 A chemical precipitator was used to precipitate vibro cholerae to the substrate of water.In the buffer solution,specific weak-alkali growth conditions were maintained to restrain the growth of other bacteria,so as to rapidly enhance the detectable rate for vibro cholerae.After the sample was centrifuged,sodium carbonate as the medium and ferrous sulfate as the precipitator were added at a certain ratio to it.After removing lucid water and adding carbonate as the buffer solution,the same amount of alkaline peptone was added at twice the concentration to proliferate,separate and identify vibro cholerae.Second proliferation was not necessary in this method,so the detection period was shortened by half compared with conventional methods.The cost of reagent was also reduced.Being simple,accurate and practical,the method was featured by its detection sensitivity that was higher than those of conventional methods.更多还原