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饱和硫酸铵法提取血清中IgG最佳条件的研究
Study of optimal condition on extraction and purification IgG from bovine serum by saturated ammonium sulphate method
【摘要】 利用饱和硫酸铵法对牛血清中的免疫球蛋白进行分离提取,得到免疫球蛋白的粗提物,研究不同单因素对IgG质量浓度及IgG纯度的影响,确定最佳提取参数如下:提取温度为50℃,静置时间为50min,血清-PBS比例2︰1(体积比),缓冲溶液pH值7.4,离心机转速4500r/min。
【Abstract】 In this artical,the Ig was isolated from bovine serum by the neuter salt methods using saturated ammonium sulphate,and then got the crude extraction of Ig.the relationship between the content of IgG,purity of IgG and different singal factors were researched,the results showed that separating at 50 ℃?4 500 r/min,resting for 50 min,mixing serum and PBS(pH7.4) at 2:1 were the best parameters.,
【关键词】 饱和硫酸铵法;
提纯;
血清;
IgG;
影响因素;
【Key words】 saturated ammonium sulphate method; purify; IgG; infection factor;
【Key words】 saturated ammonium sulphate method; purify; IgG; infection factor;
【基金】 国家自然科学基金资助项目(20266005)
- 【文献出处】 中国乳品工业 ,China Dairy Industry , 编辑部邮箱 ,2006年01期
- 【分类号】TS252.1
- 【被引频次】41
- 【下载频次】877