【摘要】 利用RT-PCR的方法从红树林海莲中克隆丙二烯环化氧化酶基因(AOC),并进行序列测定和BLAST程序进行同源序列分析。从RT-PCR得到的产物,其核苷酸序列与GeneBank中海莲丙二烯环化氧化酶基因AO(CBAB21610)的核苷酸序列完全相同。AOC基因通过中间载体pBS-AOC,pJIT60-AOC,最后克隆到植物表达载体pGreenII0229上,得到双35S的pGreenII0229-AOC植物表达载体。AOC基因和ipt基因通过中间载体pBS-AOC,pBS-ipt,pBS-AOC-ipt,pJIT60-AOC-ipt;最后将AOC和ipt基因以转录融合的方式克隆到植物表达载体pGreenII0229上,得到具有双35S启动子的AOC,ipt双基因转录融合的植物表达载体pGreenII0229-AOC-ipt。更多还原

【Abstract】 AOC gene was isolated from Bruguiera sexangula (Lour.) Poir by RT-PCR, which has been identified to be homologous to the gene NO: BAB21610 in GeneBank. The AOC, mediated by pBS-AOC and pJIT60-AOC, was cloned onto the plant expression vector pGreenII0229 to construct pGreenII0229-AOC (2S0229-AOC) with double 35S. Mediated by pBS-AOC, pBS-ipt, pBS-AOC-ipt, pJIT60-AOC-ipt, the AOC gene and the ipt gene were fused and transcribed onto the pGreen0229, and the pGreenII0229-AOC-ipt (2S0229-AOC-ipt) with a double 35S promoter was constructed. The isolation of the AOC gene and the construction of the expression vectors are valuable for using the AOC and ipt genes to improve plant traits.更多还原