【摘要】 目的研究各种昆虫抗菌肽对肿瘤细胞的作用效果。方法对家蝇(Muscadomestica)幼虫、蛹及德国蜚蠊(Blattellagermanica)、美洲蜚蠊(Periplanetaamericana)若虫采用针刺感染大肠杆菌诱导产生抗菌肽,经研磨、层析提取其抗菌肽,并通过流式细胞仪测定各种抗菌肽对人髓样白血病细胞(K562)的作用。结果①抑菌圈显示所提取的各种抗菌肽均有一定的抑菌作用。所提抗菌肽浓度:家蝇幼虫6.51mg/ml,家蝇蛹4.08mg/ml,美洲大蠊6.93mg/ml,德国小蠊6.71mg/ml。②家蝇幼虫和蛹的抗菌肽对K562有一定的杀伤作用,而美洲蜚蠊和德国蜚蠊不仅不能杀伤K562,在某种程度上还有减少其死亡和促进其生长的作用。结论各种昆虫抗菌肽由于结构不同,因而功能也太不同。更多还原

【Abstract】 Objective To study the anticancer activity of antimicrobial peptides isolated from Musca domestica,Blattella germanica and Periplaneta americana. Method The three insects were infected with Esherichia coli by a sting,then the antimicobial peptides were obtained from the insects.The peptides were isolated by grinding of the insect with buffer. The peptides were then isolated by gel filtration chromatopgraphy through a Sephadex G-50 column. The anticancer effect of the peptides on K562 cells was analyzed by flow cytometery. Result All of peptides could inhibit the growth of Escherichia coli. The concentration of peptides isolated are Musca domestica larva 6.51 mg/ml,Musca domestica pupa 4.08 mg/ml, Periplaneta americana 6.93 mg/ml,and Blattella germanica 6.71 mg/ml. The peptides from Musca domestica demonstrated a killing effect on K562 cells,while peptides isolated from the two cockroach could not kill K562 cells but promot the growth of K562 cells to a certain extent. Conclusion Some antimicrobial peptides from insects like fly could kill cancer cell, but some others may have an opposite effect.更多还原