【摘要】 目的应用不同剂量的¹³⁷Csγ-射线（0～35Gry）照射ACD-B配方保存的新鲜全血,观察照射前后T淋巴细胞亚群[CD3+（总T）、CD4+（辅助性T）、CD8+（抑制性T）]的变化。方法将10份每份容量为200ml的ACD-B配方保存的新鲜全血（保质期21d）分为4组,分为用15Gry、25Gry、35Gry不同剂量г-射线照射的实验组及未照射的对照组,于采血后立即进行照射,用流式细胞仪检测照射前后及不同照射剂量之间T淋巴细胞亚群的变化。结果不同剂量的¹³⁷Csγ-射线照射新鲜全血,照射前后T淋巴细胞亚群百分数（CD3+、CD4+、CD8+）的变化无显著性差异。结论15～35Gry的¹³⁷Csγ-射线照对新鲜全血中T淋巴细胞亚群CD3+、CD4+、CD8+百分比数量无改变,CD4+/CD8+比值无改变。更多还原

【Abstract】 Objective To examine the effects of whole blood ¹³⁷Cs gamma irradiation on the change of lymphocyte sub-populations（CD3+ T cell、CD4+ T cell、CD8+ T cell）. Method Ten samples of ACD-B whole blood were divided into 4 groups. Experimental groups were irradiated with gamma ray at a dose of 15, 25, and 35 Gry respectively. Control group was not irradiated. Lymphocyte subpopulations were analyzed by flow cytometry. Result Significant difference in the percentang of CD3+,CD4+,and CD8+ lymphocytes between the experimental group and the control group was not observed. Conclusion The percentage of CD3+, CD4+, and CD8+ lymphocytes and the ratio of CD4+/CD8+ in whole blood were not affected by 15～35 Gry ¹³⁷Cs gamma irradiation.更多还原