【摘要】 以剑麻的嫩芽为外植体,在N6+2,4-D2mg/L+6-BA2mg/L+NAA0.5mg/L培养基上诱导出愈伤组织,再在MS+6-BA2mg/L+NAA0.05mg/L培养基上诱导不定芽和增殖培养,不定芽在MS培养基上诱导生根形成完整植株,生根率达100%。将小苗移栽至基质配比为椰糠∶河沙等于1∶1的育苗盆中,成活率达100%。更多还原

【Abstract】 Calli were induced on N6 medium supplemented with 2,4-D 2.0 mg/L, 6-BA 2.0 mg/L and NAA 0.5 mg/L by using auxiliary buds of Agave sisalana Perrine ex Engelmann as explants. Adventitious buds were induced and proliferated on the MS at the presence of 6-BA 2.0 mg/L + NAA 0.05 mg/L. The adventitious buds cultured on the MS medium were induced to root to form rooted plantlets with the rooting rate being 100%. The rooted plantlets survived by 100% when transplanted onto containers for culture on the medium containing coir and sand at a ratio of 1:1.更多还原