【摘要】 目的了解早期应用双歧杆菌对非肥胖糖尿病(NOD)小鼠糖尿病发病的影响。方法将4周龄雌性NOD小鼠随机分为两组:实验组(20只)给予双歧杆菌0.25mg/g体重/天(制备成活菌悬液0.5ml),对照组(20只)给予等量的磷酸盐缓冲液(PBS)。4周龄开始给药至30周龄,每周测体重,于15周龄时各组分别处死6只小鼠,取胰腺组织HE染色进行胰岛炎评分;其余小鼠30周龄时观察发病率,同时观察胰岛炎,脾脏T细胞亚群变化。结果双歧杆菌组糖尿病发病率28.57%,明显低于对照组78.57%(P<0.05),发病时间也显著延缓,胰岛炎程度明显减轻,残存胰岛数目高于对照组;脾CD4+、CD8+T细胞亚群双歧杆菌组明显低于对照组(CD4+T细胞P<0.01,CD8+T细胞P<0.05)。结论双歧杆菌对NOD小鼠1型糖尿病发病有预防和延迟的作用,其机制可能与调节小鼠CD4+、CD8+T细胞亚群有关。更多还原

【Abstract】 Objective To study whether bifidobacterium can prevent the onset of type 1 diabetes in female non-obese diabetic（NOD） mice.Methods 40 female NOD mice aged four weeks were divided into two groups.Experiment group（n=20）was orally administered bifidobacterium（0.25mg/gBM/d,prepared viable organism suspension 0.5ml）,and the contrast group （n=20） was given PBS by the same regimen.Mice aged four weeks were administered until 30 weeks of age.At the age of fifteen weeks,the degree of insulitis was observed;The incidence of diabetes was detected at age of thirty weeks,at the same time,to observe insulitis and change of T cell subsets of spleen.Results Treatment with bifidobacterium reduced the total incidence of diabetes（28.57% Vs 78.57%,P<0.05） and delay its onset compared with control group.The level of insulitis were lessen than control group,but the number of survival areas of Langerhans were higher than control group;CD₄+、CD₈+T cell subsets of spleen were lower obviously than control group.（CD₄+ T cell P<0.01,CD₈+T cell P<0.05）.Conclusions The data demonstrated the preventive effects of bifidobacterium on the onset of type 1 diabetes and the severity of insulitis in female NOD mice.The mechanisms of these effects may be related to the change of CD₄+、CD₈+T cell subsets.更多还原