【摘要】 目的:探讨泌乳素诱导蛋白(PIP)mRNA作为标志检测乳腺癌患者外周血中微转移的可能性。方法:用逆转录-聚合酶链反应(RT-PCR)法,检测69例乳腺癌患者外周血中PIPmRNA表达情况,另抽取28例乳腺良性疾病患者和10例健康志愿者外周血标本作为对照组。结果:69例乳腺癌患者外周血中PIPmRNA有21例表达,阳性率为30.4%,对照组中PIPmRNA表达均为阴性。两组比较差异有统计学意义,P<0.05。PIPmRNA的阳性表达与患者的淋巴结转移状况差异有统计学意义,P<0.05;而与患者的年龄、原发肿瘤大小、分期、病理类型、ER、PR等差异均无统计学意义,P>0.05。结论:PIPmRNA可能作为标志来检测乳腺患者外周血中微转移。更多还原

【Abstract】 OBJECTIVE:To explore the clinical significancer of the detection of breast cancer micrometastases in peripheral blood by using PIP(prolactin-inducible protein)mRNA reverse transcription-polymerase chain reaction(RT-PCR).METHODS:PIP mRNA level in peripheral blood was detected in 69 patients with breast cancer,28 patients with breast benign lesions and 10 heathly volunteers as conrols by RT-PCR.RESULTS:The potisve rate of PIP mRNA expession was 30.4%,no positive PIP mRNA was found in peripheral blood of 28 patients with breast benign lesions and 10 heathly volunteers.There was a significant difference between the two groups.PIP mRNA expession was correlated with axillary lymph nodes status.There were no significant differences between any of other clinical parameters(age,tumor size,TNM stage,histopathlogy types,estrogen receptor and progesterone receptor).CONCLUSION:PIP mRNA may be a tumor marker in detecting cancer cells in peripheral blood.更多还原