【摘要】 目的:探讨人端粒酶逆转录酶(human telomerase reverse transcriptase,hTERT)和Ki-67的表达与中晚期宫颈癌放射敏感性的关系。方法:应用免疫组化方法检测72例中晚期宫颈癌组织hTERT和Ki-67的表达情况。全部患者均采用单纯放射治疗,外照射用直线加速器6MV X线等中心盆腔野常规放疗DT=40~44Gy,腔内后装治疗(192Ir)每周1次6Gy,A点参考剂量42Gy。结果:hTERT和Ki-67的表达指数分别分布为56·4%~95·2%和4·8%~52·4%,平均值分别为75·8%和26·6%。hTERT和Ki-67的表达强度与放射敏感性相关,随着hTERT和Ki-67表达的增高,其放射敏感性有增高趋势。Logistic多因素回归分析,筛选出对放射敏感性有影响的因素依次为:hTERT、肿瘤的分化程度和肿瘤的体积。结论:hTERT和Ki-67表达与中晚期宫颈癌的放射敏感性呈正相关。更多还原

【Abstract】 OBJECTIVE:To investigate the relationship between the expressions of human telomerase reverse transcriptase (hTERT), Ki-67 antigen and the radiosensitivity of middle-late phase cervical carcinoma. METHODS: The immunohisotechemistry staining was used to examine the expressions of hTERT and Ki-67 in 72 cases of middle-late cervical carcinoma. All patients were treated with radio-therapy by using 6 MV accelerator and intracavitary afterloading therapy. The prescribed dose and a point radiation dose were 40-44 Gy/20-22f/28-30 d and 42 Gy/7f/6w, respectively. RESULTS: The expression percentages of hTERT and Ki-67 were 75.8%(56.4%-95.2%) and 26.6%(4.8%-52.4%), respectively. The expressions of hTERT and Ki-67 were correlated with the radiosensitivity of middle-late phase cervical carcinoma. Radiosensitivity was trending up with the expressions of hTERT and Ki-67. Logistic multivariate regression analysis showed that significant influencing factors of the radiosensitivity were hTERT, differentiation degree and volume of tumor of middle-late phase cervical carcinoma. CONCLUSIONS: The expressions of hTERT and Ki-67 are positively correlated with the radiosensitivity of middle-late phase cervical carcinoma.更多还原