【摘要】 目的:研究生长激素1(growth hormone1,GH1)基因T1663A多态性与结直肠癌易感性的关系。方法:在江苏省进行了一个病例-对照研究(结直肠癌患者315例,人群对照439名),调查研究对象的生活习惯,抽取静脉血,提取白细胞DNA,采用PCR-RFLP检测研究对象的GH1T1663A基因型。结果:1)GH1T/T、T/A和A/A基因型分布频度在结直肠癌组分别为42.2%、46.7%和11.1%,对照组分别为38·1%、45·9%和16·0%,两组差异无统计学意义,χ2MH=3·907,P=0·142。但在调整性别、年龄、吸烟和饮酒习惯后,A/A基因型者与T/T基因型者相比,发生结直肠癌的危险性显著降低(OR=0·88,95%CI:0·78~0·99,P=0·0287)。2)多因素分析结果显示,饮酒者患结直肠癌的危险性显著增高(OR=1·96,95%CI:1·34~2·86,P=0·0005),A/A基因型与降低结直肠癌的危险性有关,而吸烟与增加或降低结直肠癌的危险性无显著相关。3)GH1基因多态与吸烟、饮酒相互作用的分层分析发现,在不吸烟者中,GH1A/A基因型者与T等位基因型者相比,发生结直肠癌的危险性显著降低(性别和年龄调整OR=0·50,95%CI:0·27~0·93);在不饮酒者中,GH1A/A基因型者发生结直肠癌的调整OR为0·56(95%CI:0·32~0·99)。结论:T1663A GH1基因的A/A基因型可降低结直肠癌易感性,特别是在不吸烟和不饮酒者中。更多还原

【Abstract】 OBJECTIVE:To study the relationship between genetic polymorphisms of growth hormone 1 (GH1) T1663A and the susceptibility of colorectal cancer (CRC). METHODS: A case control study was conducted with 315 patients with CRC and 439 population-based controls in Jiangsu Province, China. The epidemiological data were collected, and DNAs of peripheral blood leukocytes were obtained from all of the subjects. GH1 genotypes were detected by PCR-RFLP. RESULTS: 1) The frequencies of the GH1 T/T, T/A and A/A genotypes were 42.2%, 46.7% and 11.1% in colorectal cancer cases, and 38.1%, 45.9% and 16.0% in controls, respectively. Compared with individuals who had GH1 T/T genotype, individuals who had GH1 A/A genotype were at a decreased risk of developing colorectal cancer (sex-, age-, smoking-and alcohol drinking-adjusted OR=0.88, 95%CI: 0.78-0.99, P=0.029). 2) Smoking was not associated with the risk of colorectal cancer, whereas alcohol drinking was associated with the increased risk of colorectal cancer (sex-, age-, smoking- and GH1 genotype-adjusted OR=1.96, 95%CI: 1.34-2.86). 3) Among non-smokers, individuals who had GH1 A/A genotype were at a decreased risk of developing colorectal cancer (sex-, age-adjusted OR=0.50, 95%CI: 0.27-0.93) compared with individuals who had GH1 T allele. Among non-drinkers, individuals who had GH1 A/A genotype also were at a decreased risk of developing colorectal cancer (sex-, age-adjusted OR=0.56, 95%CI: 0.32-0.99) compared with individuals who had GH1 T allele. CONCLUSION: The results show that the GH1 gene T1663A polymorphism can influence susceptibility of colorectal cancer, particularly in non-smokers or non-drinkers.更多还原