【摘要】 目的:观察应用5-氟尿嘧啶术前化疗对胃癌细胞凋亡和增殖的影响。方法:30例进展期胃癌患者术前应用5-氟尿嘧啶,周围静脉给药,750mg/d,连用5d后手术。对比分析术前胃镜活检及术后切取肿瘤组织细胞凋亡和增殖情况,分别应用TUNEL标记和免疫组化方法。结果:化疗后胃癌细胞凋亡总指数为(10·1±5·3)%,较化疗前凋亡总指数(6·9±3·0)%显著增高,P=0·026。高中分化腺癌组、肿瘤直径<4cm组、未浸及浆膜层组化疗前后凋亡指数差异明显。化疗后增殖总指数为(48·7±13·4)%,较化疗前增殖总指数(58·4±14·8)%显著降低,P=0·0035。化疗前后各组均差异有统计学意义。结论:5-氟尿嘧啶术前静脉化疗可诱导胃癌细胞凋亡,并有效抑制胃癌细胞增殖。5-氟尿嘧啶对分化程度高的胃癌诱导凋亡作用明显。更多还原

【Abstract】 OBJECTIVE:To investigate the effect of preoperative chemotherapy with 5-fluorouracil on apoptosis and proliferation activity of gastric carcinoma cells. METHODS: Continuous intravenous administration of 5-Fluorouracil at 750 mg/day for 5 days were carried out preoperatively in thirty patients with advanced gastric adenocarcinoma. Apoptosis and proliferation of cancer cells were comparatively studied between pre-chemtherapeutic biopsy and post-chemtherapeutic resected carcinoma tissues with TUNEL and the immunohistochemical method respectively. RESULTS: The general apoptosis indexes before and after themotherapy were (6.9±3.0)% and (10.1±5.2)% respectively. Significant difference was found between them, P=0.026. Significant differences were found before and after chemotherapy in well differentiated gastric adenocarcinomas, tumors with maximum diameter less than 4 centimeters and tumors without serosa invasion. The general proliferation index after chemotherapy was (48.7±13.4)%, which was significantly lower than that before chemotherapy (58.4±14.8)%, P=0.003 5. Significant differences in proliferation index before and after chemotherapy were found in all groups. CONCLUSIONS: Preoperatively intravenous chemotherapy with 5-fluorouracil can induce apoptosis and inhibite proliferation effectively in gastric cancer. In particular, the effect on apoptosis is significant in well-differentiated gastric cancer.更多还原