【摘要】 目的:探讨双自杀基因CD和TK对K562细胞的体内外抑制作用及前体药物对肿瘤的杀伤作用。方法:将目的基因转染入K562细胞,MTT法观察细胞在体内外的增殖状况及5-FC/GCV对转染细胞的杀伤作用,电子显微镜观察其超微结构变化。将K562/CDgly TK和K562细胞接种于裸鼠皮下,观察各种肿瘤细胞在体内的成瘤情况及对前体药物治疗的敏感性。结果:单独使用GCV或5-FC对K562及K562/CDgly TK细胞产生明显的杀伤作用,联合应用该2种药物对肿瘤细胞的杀伤作用更强。将K562细胞和K562/CDgly TK细胞接种于小鼠皮下后小鼠成瘤率为100%,GCV或5-FC可明显抑制裸鼠体内的肿瘤形成,联合应用GCV和5-FC治疗K562/CDglyTK细胞在小鼠体内形成的肿瘤,较单独应用GCV和5-FC及对照组小鼠形成的肿瘤体积明显缩小,生存期也明显延长。结论:双自杀基因在体内外对K562细胞均有明显的抑制作用,可增加前体药物GCV和5-FC对瘤细胞的杀伤率。更多还原

【Abstract】 OBJECTIVE:To study the killing effect of double suicide genes system on K562 cells in vivo and in vitro.METHODS: CDglyTK gene was transfected into K562 cells by using lipofectamine.K562 cells were infected with viral supernant.K562/CDgly TK cells were treated with 5-FC and/or GCV.The mice were divided into three groups randomly: tumor formation group,tumor inhibition group and tumor therapy group.Each mouse was implanted with(K562/CDgly) TK cells or K562 cells.RESULTS: The killing effect of 5-FC and GCV in combination on K562/CDglyTK was more effective than that of using 5-FC or GCV alone.In vivo study,the rate of tumor formation was 100%.Double suicide genes could suppress tumor formation of K562/CDglyTK cells.After the mice were treated with double suicide genes,the median tumor volume of mice implanted with K562/CDglyTK cells decreased obviously compared with the control group.Their median survival was significantly prolonged.CONCLUSIONS: Double suicide genes are more effective for killing effect on K562 cells in vivo and in vitro.It may be applicable for clinical gene therapy.更多还原