【摘要】 目的研究类风湿关节炎(RA)患者血清、关节滑液中调节活化正常T细胞表达和分泌因子(RANTES)及单核细胞趋化蛋白1(MCP-1)表达水平,探讨其在RA致病中的作用。方法用酶联免疫吸附法(ELISA)测定27例类风湿关节炎患者血清及病变膝关节滑液中RANTES、MCP-1水平,并与外伤截肢者对照。结果RA患者血清及滑液中RANTES、MCP-1水平明显高于对照组,且RA滑液中RANTES水平高于血清。结论以上研究结果提示RA病变关节组织是RANTES产生的主要部位。更多还原

【Abstract】 Objective To investigate the expression of activation normal Tcell expressed and secreted(RANTES)and monocytechemoattracta protein1(MCP-1) in serum and synovial fluid of patients with rheumatoid arthritis(RA)and to identify its pathological role in RA.Methods The serum and synovial fluid of the pathologic knee joints were obtained from patients with RA,and samples fromleg traumatic amputees were used as the control group.Levels of RANTES and MCPˉ1protein in serum and synovial fluid were measured by enzymeˉlinked immunosorbent assay(ELISA).Results Levels of RANTES,MCPˉ1protein in both serum and synovial fluid of patients with RA were significantly higher compared with corresponding samples of the control group.In addition,the expression of RANTES protein in synovial fluid was also markedly increased compared with that in serum of patients with RA.Conclusion All the results suggest that the pathological arthrosis are the main positions to produce RANTES.更多还原