【摘要】 目的建立大鼠血浆中淫羊藿苷的检测方法。方法采用RP-HPLC法测定大鼠血浆中的药物浓度,色谱柱为Lichrospher C18(4.6mm×250 mm,5μm),流动相为甲醇-水-冰醋酸(45∶55∶0.5 v/v),检测波长为270 nm,流速为1 m l.m in-1。结果在1~96 mg.L-1范围内淫羊藿苷峰面积与浓度线性关系良好(r=0.9993),最低检测浓度为0.03 mg.L-1,绝对回收率为85.3%~96.6%,准确度为94.3%~101.2%,日内、日间RSD均小于10%。结论本测定方法灵敏、准确、简便,适合于淫羊藿苷的药代动力学研究。更多还原

【Abstract】 Objective To develop a simple,sensitive and rapid method for the determination of Icariin in rat plasma.Methods The plasma concentration of Icariin was determined by RP-HPLC method,the analysis involved a 250 mm × 4.6mm column packed with C18(5 μm),the mobile phase consisted of methyl alcohol-water-glacialacetic acid(45:55:0.5),the detection was performed at UV 270nm.Results A good linearity was obtained in the range of 1～96 mg/L(r=0.9993),the recovery was between 85.3~96.6%,the accuracy was between(94.3%)~101.2%.The relative standard deviations of within-day and between day were less than 10%, the minimal detectable concentration in rat plasma was 0.03 mg/L.Conclusion The result demonstrated that the method was sensitive,accurate and simple for the determination of Icariin in rat plasma,it was suitable for the pharmacokinetics study of Icariin.更多还原