【摘要】 用细胞色素B基因PCR-RFLP方法对阿胶原料进行鉴定。提取马、牛、驴3种动物干皮DNA,PCR扩增细胞色素B基因保守区域的359 bp片段,用限制性内切酶HinfⅠ和HaeⅢ酶切,采用琼脂糖凝胶电泳获得了DNA指纹图谱,根据获得的DNA指纹图谱判定样品所属物种。该方法不仅简便,还可以100%准确鉴定阿胶原料皮样所属物种来源,适合作为常规技术应用于阿胶原料鉴定。更多还原

【Abstract】 This study describes an investigation into the use of a PCR-RFLP technique for the identification of the raw material of the traditional Chinese medicine ejiao.The technique was used to generate DNA fingerprints for 3 animal species involved in ejiao production,namely donkeys,horses and cattle,by amplifying a 359 bp region within the cytochrome b gene and digesting the amplified product using restriction enzyme Hinf I and Hae Ⅲ.All three species could be discriminated using the two restriction enzymes.The results of this study suggest that the CytB PCR-RFLP method shows promise as a routine analytical tool for species testing of the raw material in ejiao production.更多还原