The effects of several bio-fungicides on the control of cucumber downy mildew were studied. The results show that control effects of wettable powder which was made of Bacillus subtilis Y1336 were 71.78% and 82.75% with the concentration of 1.25 × 106 cfu/mL and 3.3 × 106 cfu/mL, respectively. and that of 0.3% matrine AS( an abstract from Sophora flavescens) were 66.83% and 77.04% with the concentration of 22.5 g a.i./ha and 50.0 g a.i./ha, respectively. These bio-fungicides were no harm to biology.