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不同生育阶段黄瓜菌核病菌对几种三唑类杀菌剂的敏感性
Susceptivity of Sclerotinia sclerotiorum to Triazole Fungicides at Different Growth Stages
【摘要】 采用菌丝生长速率法测定了6种三唑类杀菌剂和4种其他杀菌剂抑制黄瓜菌核病菌Sclerotinia sclerotiorum(L ib.)de Bary菌丝生长的毒力,同时测定了其对菌核形成及萌发的抑制作用。结果表明,甲基硫菌灵抑制菌丝生长的毒力最低,EC50值为65.75μg/mL;己唑醇和戊唑醇的抑制活性较高,EC50值分别为0.09和0.16μg/mL,毒力分别是甲基硫菌灵的763.7和423.4倍;其次是丙环唑、氟硅唑、腈菌唑,毒力是甲基硫菌灵的137.3~286.6倍;再次为三唑酮,毒力仅为甲基硫菌灵的20.6倍;腐霉利、菌毒清抑制菌丝生长的毒力较低,EC50值均大于10μg/mL。在EC50值浓度下,所有供试药剂对菌核形成及其数量均没有影响,但三唑类杀菌剂对菌核单重影响较大,各处理所形成的菌核单重降低率均在70%以上;而当药剂浓度在10μg/mL以下时,己唑醇等三唑类杀菌剂对菌核萌发均没有影响。
【Abstract】 The inhibition bioactivities on mycelial growth of 6 triazole fungicides and 4 others against Sclerotinia sclerotiorumLib.de Bary were determined by mycelial growth rate method in lab,andexamed their effects on sclerotium formation and sclerotium germination.The results showed thatthiophanate-methyl had the weakest inhibitory effect on mycelial growth,its EC50 value was65.75 μg/mL.Hexaconazole and tebuconazole had more significant inhibitory effects,their EC50 values were 0.09 μg/mL and 0.16 μg/mL,relative toxicity coefficient to thiophanate-methyl were 763.7 and 423.4 times,respectively.Secondary were propiconazol,mvclobutanil and flusilazole,which relative toxicities ranging from 137.3 to 286.6 times of thiophanatemethyl.Triadimefon had lower inhibition,its relative toxicity was20.6 times of thiophanate-methyl.TheEC50 values ofprocymidone andJunduqing were more than 10 μg/mL.At the concentration of EC50,all the testedfungicides did not affect the number ofsclerotium.But the weight of sclerotium fell more than70%after treated with triazole fungicides.All the triazole fungicides had no effect on the germination of sclerotium below the concentration of 10 μg/mL.
【Key words】 triazole fungicide; Sclerotinia sclerotiorum(Lib.)de Bary; mycelial growth; sclerotium germination; bioactivity;
- 【文献出处】 农药学学报 ,Chinese Journal of Pesticide Science , 编辑部邮箱 ,2006年02期
- 【分类号】S436.421
- 【被引频次】16
- 【下载频次】177