【摘要】 30只孕鼠随机分成正常对照组(Con,n=10)、阳性对照组(P,n=10)和实验组(T,n=10)。实验组大鼠每天按100mg/kg体重灌喂黄芪多糖(astragaluspolysaccharide,APS),直至实验结束,而对照组大鼠灌喂相应体积的生理盐水(physiologicalsalinesolution,PSS)。孕鼠于产后72h分别用灭菌PSS和50μg大肠杆菌(Escherichiacoli)内毒素经乳头管灌注到大鼠第四对乳腺内。灌注后24h处死大鼠,取乳腺组织固定,进行组织学观察,观察显示P组的大鼠乳腺组织破坏得最为严重。P组大鼠乳腺组织中白细胞介素-2(IL-2)浓度与正常对照组相比显著降低(P<0.05);T组大鼠乳腺组织中IL-2浓度与P组相比显著升高(P<0.05)。与正常对照组相比,P组大鼠外围血CD4+T细胞数量显著下降(P<0.05),CD8+T细胞数量显著升高(P<0.05),CD4+/CD8+水平显著下降(P<0.05);而T组大鼠外周血CD4+T细胞数量、CD8+T细胞数量及CD4+/CD8+水平均无显著变化(P>0.05)。结果表明,灌喂APS可以减轻炎症对T淋巴细胞增殖反应的抑制,改善感染导致的机体细胞免疫功能下降,对内毒素诱发的大鼠实验性乳腺炎有一定的保护作用。更多还原

【Abstract】 Thirty pregnant SD rats were randomly divided into normal control group (n =10), positive control group (n =10) and treatment group (n =10). Astragalus polysaccharide (APS, 100 mg / kg / d) or physiological saline solution (PSS) was administrated by mouth to the rats of the treatment group and control group until they were euthanatized. Either sterile PSS or 50 μg Escherichia coli endotoxin was inoculated into the fourth (abdominal) mammary gland via the teat duct 72 h after parturition. Exactly 24 h after inoculation, the rats were euthanatized and the mammary glands were removed and fixed for histopathologic evaluations. Histopathologic examination of mammary gland revealed that the mammary glands of positive control group had more severe pathologic lesion than that of the treatment group. There was significant increase (P <0.05) of interleukin-2 (IL-2) in mammary gland of treatment group compared with those of positive group. Compared with the normal group, CD4+ T lymphocyte subsets and CD4+/CD8+ ratio decreased remarkably in the peripheral blood of the positive control group. While CD4+ T lymphocyte subsets and CD4+/CD8+ ratio had no remarkable change in the peripheral blood of the treatment group. The results demonstrate that astragalus polysaccharide can promote proliferation of T lymphocyte and has protective effect on rat experimental mastitis induced by endotoxin.更多还原