【摘要】 实验运用Northernblot检测和生理生化分析等技术研究热胁迫对大豆(Glycinemax)品种花荚离层细胞的HSP70基因表达、能量供应及花荚脱落的影响。结果显示,热胁迫能够提高所有6个供试大豆品种花荚离层细胞的HSP70基因表达水平、线粒体及其蛋白含量以及细胞色素氧化酶表达量,而不同程度地降低了所有供试品种的花荚脱落率。结果说明,大豆花荚离层细胞HSP70基因的高效表达可能是通过有效地改善花荚离层细胞的能量供应,从而达到抑制花荚脱落的目的。更多还原

【Abstract】 The effects of heat stress(HS) on HSP70 gene expression, energy supply in abscission zone of flower-pod and the abscission rate of flowers-pods in soybean (Glycine max) cultivars were analyzed by Northern blot and physiological and biochemical assay. The results showed that HS could lead to the increases of HSP70 gene expression level, mitochondria and their protein contents, and cytochrome oxidase content in abscission zone of flowers-pods, but the decrease of the abscission rate of flowers-pods in all six soybean cultivars comparison with their control groups with normal temperature treatment. The results suggested that the overexpression of HSP70 gene caused the increases of mitochondria and their protein contents and cytochrome oxidase content, which improved enhancement of energy supply in abscission zone of flowers-pods so that this inhibited the abscission of flowers-pods of soybean plants. This will be of importance for reducing abscission rate of flowers-pods and increasing seed yield in soybean by genetic engineering approach.更多还原