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奥利亚罗非鱼(♀)×鳜(♂)杂交后代及其双亲的染色体核型分析

Analysis of Chromosome Karyotypes of Oreochromis aurea(♀), Siniperca chuatsi(♂) and Their Offspring

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【作者】 曹丽萍丁炜东贾永义夏德全吴婷婷

【Author】 CAO Li-ping, DING Wei-dong, JIA Yong-yi ,XIA De-quan, WU Ting-ting (Freshwater Fisheries Research Center , Chinese Academy of Fishery Science, Wuxi 214081, China)

【机构】 中国水产科学院淡水渔业研究中心中国水产科学院淡水渔业研究中心 无锡214081无锡

【摘要】 采用人工授精法以奥利亚罗非鱼(Oreochromisaurea)为母本,鳜(Sinipercaahuasti)为父本,获得了能正常发育的后代。利用微量全血培养法对奥利亚罗非鱼、鳜及它们杂交后代的染色体数目和核型进行了分析,结果表明,奥利亚罗非鱼染色体数!"2n=44,核型公式为6sm+24st+14t,染色体臂数(NF)=50;鳜鱼为2n=48,核型公式为8sm+4st+36t,NF=56;杂交后代为2n=44,核型公式为6sm+26st+12t,NF=50。经对比分析,杂交后代与奥利亚罗非鱼的核型相似。

【Abstract】 The progenies of the distant hybridization between Oreochromis aurea (♀) and Siniperca ahuasti (♂) were successfully obtained through years of research. And their karyotypes were studied by half-micro blood lymphocytic cell cultivation. The result indicated that the diploid chromosome number of O. aurea (♀) was 2n =44, and karyotype formula was 6sm+24st+14t, arm number (NF) =50; the diploid chromosome number of S. chuatsi (♂) was 2n =48, and karyotype formula was 8sm+4st+36t, NF=56; the diploid chromosome number of their progenies was 2n =44, chromosome formula was 6sm+26st+12t, NF=50. The results showed the karyotypes between O. aurea (♀) and progenies were similar by comparison.

【基金】 国家自然科学基金项目(No.30371116);国家“十五”攻关专题(No.2004BA526B0110);国家高技术研究与发展计划(863)项目(No.2001AA243061)资助
  • 【文献出处】 农业生物技术学报 ,Journal of Agricultural Biotechnology , 编辑部邮箱 ,2006年02期
  • 【分类号】S917.4
  • 【被引频次】25
  • 【下载频次】282
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