【摘要】 研究环境低温诱发肉鸡肺动脉高压综合征(PHS)过程中肺小动脉壁c-junmRNA的表达变化,从而确定原癌基因c-jun在环境低温诱发肉鸡PHS过程中的参与作用,为肉鸡PHS发生机制的研究提供基础。160只雄性AA商品代肉鸡15日龄随机分为对照组(22±1.5)℃和低温组(11±2)℃。15~50日龄,每周每组随机取6只,做肺组织石蜡切片,应用原位杂交染色法进行c-junmRNA杂交,并结合图像分析法,测定对照组与低温组肉鸡肺小动脉壁原癌基因c-junmRNA的表达情况。1)低温组肉鸡PHS发生率(18.75%)极显著高于对照组(1.25%)(P<0.01);2)低温组肉鸡肺小动脉壁c-junmRNA的表达从22日龄开始较对照组明显增加(P<0.05),从29~50日龄较对照组极显著升高(P<0.01)。说明环境低温明显诱发了肉鸡肺小动脉壁c-junmRNA的表达,且c-junmR-NA的表达参与了环境低温诱发的肉鸡PHS的发生。更多还原

【Abstract】 The study was conducted to assess the expression of c-jun mRNA oncogene in the wall of pulmonary arteries in the development of pulmonary hypertension syndrome(PHS) in broilers induced by a low ambient temperature.Six broilers in control group(22±1.5)?℃ and low temperature group(11±2)?℃ were sampled every week from 15 to 50 days of age and lungs were paraffin-embedded and sectioned.The expression of c-jun mRNA oncogene in the wall of pulmonary arteries was assessed by in situ hybridization and image analysis.Finally,the incidence of PHS was calculated.The results showed that the incidence of PHS in low temperature groups was significantly higher than that in the control group and the expression of c-jun mRNA oncogene in the wall of pulmonary arteries in low temperature group was also higher than that in the control group.These demonstrated that the expression of c-jun mRNA oncogene in the wall of pulmonary arteries in broilers was induced by low ambient temperature,and facilitated the development of PHS in broilers induced by low ambient temperature.更多还原