【摘要】 为深入研究马尾松树皮提取物(PMBE)的抑癌生长作用,构建PMBE的动物药理模型,本研究通过细胞培养、细胞活力检测(MTT实验)及计算机模拟评估马尾松树皮提取物(PMBE)抑制体外培养人大肠癌LoVo细胞生长规律,建立了马尾松树皮提取物(PMBE)、胎牛血清(FBS)及处理时间(t)三因素对LoVo细胞生长抑制率的反应模型,利用该回归模型对三因素优化组合,同时就各因素单独效应及其互作效应进行了探讨。PMBE、FBS、t三因素适量水平搭配(0、1、0)可提高对LoVo的生长抑制效率,最高可达0.42,即三因子用量分别为140μg/mL、15%、48 h时效果最佳。系列数据分析结果表明,在体外处理细胞过程中可能存在抑制物效应报酬递减规律,且试验体系中多因素综合效应呈现随机非线性特点。该研究是统计学在医学科研设计、衡量和评价(D.M.E)领域的一次尝试,可为构建PMBE的动物药理模型确定药用剂量提供参考。更多还原

【Abstract】 In order to probe further into the role of PMBE inhibiting the growth of cancer,and construct the pharmacological animal model,combination of cell culture,multiple group MTT assays and computer modeling techniques was used in this present study to assess the effect of PMBE,FBS and t on the cell growth inhibition ratio（GIR） of human colorectal carcinoma LoVo cells cultured in vitro.Quadratic general rotational design at 5 levels of 3 factors was used to design the MTT assay scheme.The reaction model between the GIR（Y） and PMBE（X₁）,FBS（X₂） and t（X₃） was established.Optimal combination of the three factors,effects of single factor and interaction between them were explored respectively,and appropriate combination of different factors could suppress the cell growth effectively.By simulation with computer,the optimal combination of PMBE,FBS and t with the highest GIR 0.42 was found at 0,1,0 level,or 140?μg/mL,15% and 48?h,respectively.A law of "reward descending of inhibitor effect" and nonlinear characteristic of many factors’ integrative effect during cell treatment were first put forward.This research is a novel attempt to apply statistics principle in design,measurement and evaluation（D.M.E.） fields of medical scientific research,with reference significance in determining the dosage of candidate drug for pharmacological animal model.更多还原