【摘要】 目的为临床使用计算机辅助精子分析(CASA)系统的精液分析参数提供参考。方法对48例人类冷冻复温精液和116例临床进行丈夫人工授精(AIH)的新鲜精液体外获能前后CASA检测结果,分组进行配对资料t-检验和独立样本t-检验。结果所有实验组精子体外获能后的曲线运动速度(VCL)、直线运动速度(VSL)、平均路径速度(VAP)、精子头侧摆幅度(ALH)和鞭打频率(BCF)显著提高(P<0．01);除冻存精液精子体外获能后的运动前向性(STR)和头部椭圆度外,其余各组精液精子获能后的STR、直线性(LIN)和头部椭圆度显著下降(P<0,01)。行AIH治疗的精液标本,无论获能前后,已孕组精子VAP、LIN和头部椭圆度显著高于未孕组(P<0．05),而BCF和头部面积低于未孕组(P<0.05);获能前两组的(a+b)级精子百分率无显著性差异,获能后差异显著(P<0．05)。结论体外获能处理可显著提高精子的各种运动速度,但精子运动的前向性和直线性降低;精液常规分析结果中的精子运动等级和速度不直接反映精子的受精能力。更多还原

【Abstract】 Objective To understand the sperm parameters before and after capacitation using Computer-Assisted Sperm Analysis (CASA). Methods Using Paired-samples T test and Independent-samples T test, comparison of sperm CASA parameter before and after capacitation in 48 freeze-thawed and 116 fresh human sperm before AIH. Results The sperm Linearity (LIN) in all groups, the sperm Straightness (STR) and elongation ratio in all except freezed group were reduced after capacitation (P<0.01); But the values of sperm Curvilinear velocity (VCL), Straight line velocity(VSL), average path velocity(VAP), amplitude of lateral head displacement(ALH) and Beat-cross frequency (BCF) were increased (P<0.01). The values of sperm VAP、LIN and elongation ratio in these who had fathered a child by AIH were higher than those who failed to father a child, but the sperm BCF and head area was significantly smaller on the contrary (P<0.05); The sperm motility (a+b) was no significance in two groups (P>0.05), Conclusion The semen processing can improve the sperm parameters except LIN and STR in all groups. Successful AIH wasn’t associated with sperm motility (a+b) in semen.更多还原