【摘要】 目的研究人参皂甙Rb1体外对正常男性精子运动的影响,探讨其可能的作用机制。方法将18例正常男性经手淫获得的精子,通过上游优化法处理后,与10μmol/L人参皂甙Rb1共同孵育0．5h、1h、2h、3h、4h、5h,应用计算机辅助的精子分析系统(CASA),观察随时间延长人参皂甙Rb1对人精子各运动参数的作用。结果随着时间延长,精子活动能力均有不同程度下降；10μmol/L人参皂甙Rb1能明显提高正常男性精子的运动能力(A+B)%、精子存活率(Mot)、前向运动精子百分率、曲线运动速度(VCL)、直线运动速度(VSL)和平均路径速度(VAP),与空白对照组相比,差异均有显著性(P＜0．05)。结论随着时间延长,低浓度人参皂甙Rb1在体外能明显提高止常男性精子的存活率和运动能力,本研究为人参皂甙Rb1的临床应用提供了斯的实验基础。更多还原

【Abstract】 Objective To study the effects of ginsenoside Rb1 on human sperm mobility in vitro and to investigate its possible mechanism.Methods Using computer-assisted sperm analysis(CASA),the effects of ginsenoside Rb1 on human sperm mobility and motility in vitro were observed.The sperm obtained by masturbation and prepared by swim-up technique from 18 men were incubated in 10μmol/L ginsenoside Rb1.Measurement were carried out at 0.5h,1h, 2h,3h,4h and 5h in all specimens.Results With time to be extended,human sperm mobility was decreased and the percentage of progressive mobile sperm(A+B)%,percentage of motility (Mot),the curvilinear velocity (VCL),the straight line velocity(VSL) and average path velocity(VAP) when can be increased by 10μmol/L Rblcompared with control group,the differences were significant (P＜0.05).Conclusion Low concentration ginsenoside Rb1 can increases human sperm motility and mobility in vitro.This study provides new experimental data for clinical use of ginsenoside Rb1.更多还原